磁珠分离式样品提取微流控芯片的磁珠运动速度建模及特性研究

doi:10.3901/JME.2018.20.245

机械工程学报 ›› 2018, Vol. 54 ›› Issue (20): 245-250.doi: 10.3901/JME.2018.20.245

磁珠分离式样品提取微流控芯片的磁珠运动速度建模及特性研究

顾佳鎏, 朱鋆峰, 李松晶

哈尔滨工业大学流体控制及自动化系哈尔滨 150001

收稿日期:2018-03-20 修回日期:2018-08-20 出版日期:2018-10-20 发布日期:2018-10-20
通讯作者: 李松晶(通信作者),女,1970年出生,博士,教授,博士研究生导师。主要研究方向为液压元件与系统设计、功能流体应用、管路动态分析等。E-mail:lisongjing@hit.edu.cn
作者简介:顾佳鎏,男,1994年出生。主要研究方向为微流控磁粒子捕获系统。E-mail:1484452769@qq.com
基金资助:
国家自然科学基金（51175101）。

Modeling and Characteristics of Magnetic Bead Velocity in Microfluidic Chip for Sample Extraction with Magnetic Separation

GU Jialiu, ZHU Yunfeng, LI Songjing

Department of fluid control and automation, Harbin Institute of Technology, Harbin 150001

Received:2018-03-20 Revised:2018-08-20 Online:2018-10-20 Published:2018-10-20

摘要/Abstract

摘要： 为提高磁珠分离式生物医学样品的提取效率，采用永磁和软磁体相结合的磁场设计结构，提出一种新型磁珠分离式样品提取微流控芯片。利用静磁学和流体力学基本原理推导出流场中磁珠运动的速度模型，通过多物理场耦合仿真软件对流场中磁珠运动规律进行瞬态仿真，分析软磁体的结构和分布对微流道内磁珠的水平分速度以及竖直分速度的影响关系，计算不同设计结构所允许的磁珠完全吸附时微流道中最大允许流量。结果表明，增大软磁体的厚度可以快速减小磁珠水平运动的速度，并减小其在垂直方向上的波动，且在双软磁体作用时，软磁体的间距越大，水平方向和垂直方向的速度波动越大；间距越小，磁珠粒子速度稳定的时间越短。

关键词: 磁珠, 耦合场, 软磁体, 速度特性, 微流控

Abstract: In order to improve the separation efficiency of the biomedical samples using magnetic beads, a new magnetic separation microfluidic chip is proposed, which uses the combination of permanent magnet and soft magnet for the structure design of magnetic field. The velocity model for the motion of magnetic beads in the flow field is derived by the basic principles of magnetostatics and fluid mechanics. The motion of magnetic beads in the flow field is simulated transiently by a multiphysics-coupling simulation software. The relationship between the structure and position of soft magnetic elements on the horizontal velocity and vertical velocity of magnetic beads in microchannels is analyzed. The maximum allowable flow rate in the microchannel is calculated when the magnetic beads are completely adsorbed by different design structures. The results show that the thickness increasing of the soft magnet can rapidly reduce the horizontal speed of the magnetic bead, and reduce its fluctuation in the vertical direction. In addition, the larger the distance between the two soft magnets, the bigger the fluctuation in the horizontal and vertical directions. The smaller the distance, the shorter the time required for the magnetic bead particles to stabilize.

Key words: coupling field, magnetic Beads, microfluidic, soft magnet, velocity characteristics

中图分类号:

TG156

顾佳鎏, 朱鋆峰, 李松晶. 磁珠分离式样品提取微流控芯片的磁珠运动速度建模及特性研究[J]. 机械工程学报, 2018, 54(20): 245-250.

GU Jialiu, ZHU Yunfeng, LI Songjing. Modeling and Characteristics of Magnetic Bead Velocity in Microfluidic Chip for Sample Extraction with Magnetic Separation[J]. Journal of Mechanical Engineering, 2018, 54(20): 245-250.

参考文献

[1] ALNAIMAT F,DAGHER S,MATHEW B,et al. Microfluidics based magnetophoresis:A review[J]. Chem. Rec.,2018(18):1-18.
[2] ZHOU Ran,WANG Cheng. Microfluidic separation of magnetic particles with soft magnetic microstructures[J]. Microfluid Nanofluid,2016(20):48.
[3] SHOJI S,KAWAI K. Flow control methods and devices in micrometer scale channels[J]. Topics in Current Chemistry,2011(304):1-25.
[4] ANTFOLK M,LAURELL T. Continuous flow microfluidic separation and processing of rare cells and bioparticles found in blood[J]. Analytica Chimica Acta,2017(965):1-27.
[5] YUNG C C,CHEN M W,SHIH H L. Particles sorting in micro channel using designed micro electromagnets of magnetic field gradient[J]. Journal of Magnetism and Magnetic Materials,2016(407):209-217.
[6] PAMME N,WILHELM C. Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis[J]. Lab on a Chip,2006(6):974-980.
[7] GÓMEZ-PASTORA J,XUE Xiaozheng,KARAMPELAS I H,et al. Analysis of separators for magnetic beads recovery:From large systems to multifunctional microdevices[J]. Separation and Purification Technology, 2017(172):16-31.
[8] WU Jie,YAN Qifan,XUAN Shouhu,et al. Size-selective separation of magnetic nanospheres in a microfluidic channel[J]. Science Microfluid Nanofluid,2017(21):47.
[9] KHASHAN S A,DAGHER S,ALAZZAM A. Microfluidic multi-target sorting by magnetic repulsion[J]. Microfluidics and Nanofluidics,2018(22):64.
[10] GUSENBAUER M,SCHREFL T. Simulation of magnetic particles in microfluidic channels[J]. Journal of Magnetism and Magnetic Materials,2018(446):185-191.
[11] BANERJEE U,BIT P,GANGULY R,et al. Aggregation dynamics of particles in a microchannel due to an applied magnetic field[J]. Microfluid Nanofluid,2012(13):565-577.
[12] CAO Quanliang,LI Zhenhao,WANG Zhen,et al. Disaggregation and separation dynamics of magnetic particles in a microfluidic flow under an alternating gradient magnetic field[J]. J. Phys. D:Appl. Phys.,2018(51):195002.
[13] ZHOU Ran,YANG Qingbo,BAI Feng,et al. Fabrication and integration of microscale permanent magnets for particle separation in microfluidics[J]. Microfluid Nanofluid,2016(20):110.

[1]	闫姿姿, 李姗姗, 李铭浩, 张明航, 李铁军, 戴士杰. 交流电热流场对微纳粒子介电泳行为的影响[J]. 机械工程学报, 2017, 53(14): 202-208.
[2]	王福雨;刘伟军;赵宇辉;孔源;来佑彬. 复杂薄壁零件激光快速成形过程的热力耦合场数值模拟[J]. , 2013, 49(5): 191-198.
[3]	许少锋;汪久根. 基于压力流场的高分子在微纳通道内的横向迁移行为[J]. , 2013, 49(19): 185-191.
[4]	宋满仓;于超;张建磊;刘冲;王敏杰;杜立群. 多工位组合电极电火花成形微结构镶块试验研究[J]. , 2013, 49(15): 186-191.
[5]	宋满仓;刘莹;祝铁丽;张传赞;刘军山;刘冲. 微流控芯片注塑成型缺陷的成因与对策[J]. , 2011, 47(6): 33-38.
[6]	刘胜吉;李晓东;关涛;王建;尹必峰. 单缸柴油机油量校正器的设计计算方法[J]. , 2011, 47(14): 129-133.
[7]	张肃;陈南;李普;张建润. 基于小波迦辽金法的复杂封闭空腔结构—声耦合场分析[J]. , 2008, 44(6): 155-160.
[8]	傅新;刘素芬;阮晓东. 基于拉格朗日跟踪法的微混合器内混沌混合特性[J]. , 2008, 44(11): 64-68.
[9]	徐书洁;段玉岗;丁玉成;卢秉恒. PDMS微流控芯片复型模具的新型快速制作方法[J]. , 2007, 43(6): 105-109.
[10]	于海平;李春峰;李忠. 基于FEM的电磁缩径耦合场数值模拟[J]. , 2006, 42(7): 231-234.
[11]	谢海波;傅新;杨华勇. 基于Micro-PIV的微观流场检测技术[J]. , 2005, 41(9): 106-111.
[12]	于建群;刘军山;王立鼎;郭丽莎. 塑料电泳芯片热键合的试验研究[J]. , 2005, 41(3): 185-188.
[13]	张峰;张宏毅;周勇亮;杨渭;陈彬彬. 湿法腐蚀硅制作PDMS微流控芯片[J]. , 2005, 41(11): 194-198.
[14]	于建群;王立鼎;刘军山;郭丽莎. 塑料电泳芯片微结构模压的试验研究[J]. , 2004, 40(11): 93-97.
[15]	谢海波;傅新;刘玲;杨华勇;徐东. 基于玻璃湿法刻蚀的微流控器件加工工艺研究[J]. , 2003, 39(11): 123-129.

磁珠分离式样品提取微流控芯片的磁珠运动速度建模及特性研究

Modeling and Characteristics of Magnetic Bead Velocity in Microfluidic Chip for Sample Extraction with Magnetic Separation

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价