• CN：11-2187/TH
  • ISSN：0577-6686
机械工程学报

机械工程学报 ›› 2017, Vol. 53 ›› Issue (9): 180-186.doi: 10.3901/JME.2017.09.180

• • 上一篇    下一篇

扫码分享

立体空心血管网水凝胶支架的3D打印工艺研究*

毛伟1,2,3, 连芩1,2,3, 李涤尘1,2,3, 贾书海1   

  1. 1. 西安交通大学机械制造系统工程国家重点实验室 西安 710054;
    2. 西安交通大学快速制造国家工程研究中心 西安 710054;
    3. 西安交通大学高端制造装备协同创新研究中心 西安 710054
  • 出版日期:2017-05-05 发布日期:2017-05-05
  • 作者简介:

    毛伟，男，1990年出生。主要研究方向为3D打印与仿生制造。

    E-mail：mw510496939@mail.xjtu.edu.cn

    连芩(通信作者)，女，1971年出生，博士，副教授，博士研究生导师。主要研究方向为3D打印与仿生制造。

    E-mail：lqiamt@mail.xjtu.edu.cn

  • 基金资助:
    * 国家自然科学基金(51375371， 51323007)和国家高技术研究发展计划(863计划，2015AA020303)资助项目; 20160623收到初稿，20161209收到修改稿;

3D Printing Process for Hydro Gel with the Three-dimensional Micro Tubes to Mimic Vascular Network

MAO Wei1,2,3, LIAN Qin1,2,3, LI Dichen1,2,3, JIA Shuhai1   

  1. 1. State Key Laboratory for Manufacturing Systems Engineering， Xi’an Jiaotong University， Xi’an 710054;
    2. Rapid Manufacturing National Engineering Research Center， Xi’an Jiaotong University， Xi'an 710054;
    3. Collaborative Innovation Center of High-End Manufacturing Equipment， Xi’an Jiaotong University， Xi'an 710054
  • Online:2017-05-05 Published:2017-05-05

PDF

623

摘要：

搭建同轴喷头打印系统，研究氯化铁溶液对由同轴针头打印出的藻酸盐水凝胶空心圆管的机械强度和成型结构的影响，建立3D打印优化工艺。将质量分数为2%~4%的海藻酸钠和质量分数为4%的氯化钙通过同轴针头打印出空心圆管。将质量分数为0.25%的雾化氯化铁溶液与空心圆管反应得到有氯化铁反应的空心圆管，力学试验发现经过与0.25%氯化铁反应的空心圆管的弹性模量(0.4677 MPa±0.279 MPa(2%海藻酸钠)，2.0153 MPa±0.221 MPa(3%海藻酸钠)，11.684 MPa±0.332 MPa(4%海藻酸钠))是无氯化铁溶液反应的空心圆管的弹性模量的5.4倍(2%)，14.2倍(3%)和43.3倍(4%)，其力学强度大大增强。机械强度增强的空心圆管完成了立体网络结构和螺旋结构的复杂类血管网，通过水凝胶溶液将其封装形成生物支架。灌流实验和细胞毒性试验表明该支架具有良好的生物相容性，可有效运输和灌注细胞培养液，满足了组织工程中制造大块软组织的工程化需求。

关键词: 仿生设计, 海藻酸钠, 空心圆管, 氯化铁, 生物支架, 三维打印

Abstract:

：A coaxial nozzle printing system is built to investigate the influence of ferric chloride solution to the mechanical strength and shape structure of sodium alginate hollow tube. The optimization process of 3D printing is further determined. The concentration of 2%- 4% sodium alginate and the concentration of 4% of calcium chloride is used to print hollow tubes through the coaxial probe. The concentration of 0.25% of atomizing ferric chloride solution is used to reinforced to the calcium alginate hollow tubes. Mechanics experiment prove that the Young's modulus of the reinforced hollow tube (0.467 7 MPa±0.279 MPa(2% sodium alginate), 2.015 3 MPa±0.221 MPa(3% sodium alginate), 11.684 MPa±0.332 MPa(4% sodium alginate)) are 5.4 times(2%), 14.2 times(3%) and 43.3 times(4%) as the sodium alginate hollow tubes. The reinforced hollow tubes are used to print complex vascular networks such as lattice structure and spiral structure. The vascular networks are encapsulated with hydro gel solution to get the biological scaffold. Perfusion experiment and cell toxicity test show that the scaffolds had good biocompatibility and it is feasible to be effectively transported cells culture medium. Therefore, printed scaffolds can meet the demand of manufacturing large soft tissues in tissue engineering.

Key words: biological scaffold, bionic design, ferric chloride, micro tube, sodium alginate, 3D Printing

版权所有 © 2019 《机械工程学报》编辑部 | 京ICP备05055788号-30