Abstract: A plastic capillary electrophoresis chip is a novel kind of miniaturized device for biochemistry analysis. The major advantages of the chip include high transmittance for light; low conductivity for electrokinetic pumping and electrophoretic separation; low cost for high volume production and disposal and easily manufacturing for high aspect ratio of microchannels on the chip. A process of hot embossing for plastic capillary electrophoresis chips is studied, the relationships among the dimensions of the microchannels, the dimensions of the raised image on the template and the process conditions of hot em-bossing, including temperature, pressure and time are estab-lished by experiment, based on these a novel process of hot embossing is proposed, which lays the foundation for basic theories and techniques of structural design and manufactures of the plastic chip.

Key words: Biochips, Capillary electrophoresis chips, Hot embossing, MEMS, Microfabrication, Microfluidic chips, m-TAS