机械工程学报 ›› 2020, Vol. 56 ›› Issue (1): 196-204.doi: 10.3901/JME.2020.01.196
顾恒1,2, 连芩1,2, 王慧超1,2, 李涤尘1,2, 靳国瑞3, 崔滨1,2
收稿日期:
2019-02-23
修回日期:
2019-07-16
出版日期:
2020-01-05
发布日期:
2020-03-09
通讯作者:
连芩(通信作者),女,1971年出生,博士,副教授,博士研究生导师。主要研究方向为增材制造与仿生制造。E-mail:lqiamt@mail.xjtu.edu.cn
作者简介:
顾恒,男,1995年出生。主要研究方向为生物3D打印。E-mail:825186692@qq.com
基金资助:
GU Heng1,2, LIAN Qin1,2, WANG Huichao1,2, LI Dichen1,2, JIN Guorui3, CUI Bin1,2
Received:
2019-02-23
Revised:
2019-07-16
Online:
2020-01-05
Published:
2020-03-09
摘要: 甲基丙烯酸酐明胶(Methacrylated gelatin,GelMA)是具有光敏官能团的改性明胶,有良好的生物相容性,广泛用于构建皮肤、神经等软组织支架。然而,光照交联单一成分的GelMA,其力学性能和结构维持性较差,难以通过挤出3D打印成形生物支架。提出一种适用于挤出式打印且力学性能增强的GelMA复合凝胶,其主要成分为GelMA、明胶和海藻酸钠。黏度测试试验筛选出适合挤出3D打印的复合材料配比,3D打印工艺试验研究和分析了打印气压、喷头移动速度、喷头高度、光照条件对挤出连续性和微丝直径的影响。当喷头内径为200 μm时,GelMA复合凝胶实现顺畅出丝(φ293~1 211 μm)的条件为气压在0.05~0.25 MPa,打印速度为1~15 mm/s,喷头高度为200~600 μm,光照强度为70~272.56 mW/cm2。拉伸试验表明光照交联后,复合凝胶较之同浓度GelMA(5%,w/v)的弹性模量提高近1倍,伸长率保持一致。细胞培养试验显示GelMA复合凝胶具有良好的生物相容性,包埋在其内部的人真皮成纤维细胞培养至第7天时存活率达85%。
中图分类号:
顾恒, 连芩, 王慧超, 李涤尘, 靳国瑞, 崔滨. GelMA复合凝胶的挤出式3D打印工艺及其性能研究[J]. 机械工程学报, 2020, 56(1): 196-204.
GU Heng, LIAN Qin, WANG Huichao, LI Dichen, JIN Guorui, CUI Bin. Extrusion 3D Printing Processes and Performance Evaluation of GelMA Composite Hydrogel[J]. Journal of Mechanical Engineering, 2020, 56(1): 196-204.
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