Abstract: Cell Orientation adjustment is an important issue in micromanipulation. A non-contact cell orientation method is put forward with which the cell is made to move to desired orientation by the swirl which is produced by microfluid spraying from two tiny tubes. Both the strength and the size of the swirl can be regulated to adapt to different size and shape of cells as well as viscosity of cellular sap by changing the relative position of the tubes, their jetting velocity, their inner diameter and other parameters. This characteristic makes cell micromanipulation more convenient. The model of cell motion in swirl under micromanipulation environment is built up to simulate the swirl generation and cell movement in swirl in micro-scale. The effects of the relative position of tiny tubes and their jetting velocity on the swirl and the cell moving characteristics have been analyzed from simulation results. The experiment is also conducted to discuss the generation of the swirl and cell moving characteristics as far as the swirls in different shapes are concerned and the results show a good agreement with that of numerical simulation.

Key words: Micromanipulation, Orientation control, Rotate, Swirl, Adaptive growth, ground structure, Key Words：heat transfer channel layout, optimality criteria method, topology optimization