Advances in High-throughput Bioprinting: Strategies and Applications in Organoid Fabrication

doi:10.3901/JME.260071

Abstract

Abstract: As an advanced biomanufacturing strategy based on additive manufacturing principles, bioprinting enables the precise deposition of cells and biomaterials, providing a powerful tool for constructing complex three-dimensional biological structures. This review systematically summarizes the fundamental principles and limitations of conventional bioprinting techniques, including droplet-based, extrusion-based, and light-assisted approaches, and highlights recent advances in high-throughput bioprinting aimed at improving printing speed, parallel capacity, and automation. Furthermore, we discuss the critical applications of high-throughput bioprinting in organoid construction, such as enabling standardized and scalable production, recapitulating complex multicellular architectures, and dynamically regulating microenvironments, thereby significantly enhancing the biomimicry and reproducibility of organoids. Finally, we outline the major challenges—such as multimodal detection, the lack of standardized protocols, and the development of bioinks—and provide a forward-looking perspective on future directions, offering important insights for the clinical translation and industrialization of organoid technologies.

Key words: 3D bioprinting, high-throughput, organoid, mass production, multicellular structure, cellular microenvironment

CLC Number:

TG156

DENG Heyuan, FANG Yongcong, ZHANG Ting, XIONG Zhuo. Advances in High-throughput Bioprinting: Strategies and Applications in Organoid Fabrication[J]. Journal of Mechanical Engineering, 2026, 62(3): 70-85.

References

[1] FANG Yongcong，GUO Yuzhi，LIU Tiankun，et al. Advances in 3D bioprinting[J]. Chinese Journal of Mechanical Engineering：Additive Manufacturing Frontiers，2022，1(1)： 100011.
[2] SUN W STARLY B，DALY A，et al. The bioprinting roadmap[J]. Biofabrication，2020，12(2)：22002.
[3] DUAN Jing，FANG Yongcong，TIAN Yueming，et al. 3D bioprinting of prevascularized bone organoids for rapid in situ cranial bone reconstruction[J]. Advanced Healthcare Materials，2025，14(16)：2501376.
[4] FANG Yongcong，JI Mengke，YANG Yi，et al. 3D printing of vascularized hepatic tissues with a high cell density and heterogeneous microenvironment[J]. Biofabrication，2023，15(4)：045004.
[5] KIM J，JEONG G，YOO Y，et al. 3D bioprinting technology for modeling vascular diseases and its application[J]. Biofabrication. 2025，17(2). doi：10.1088/1758-5090/adc03a.
[6] PARK W，CHOI M，LEE J，et al. Embedded 3D-coaxial bioprinting of stenotic brain vessels with a mechanically enhanced extracellular matrix bioink for investigating hemodynamic force-induced endothelial responses[J]. Advanced Functional Materials，2025：e04276.
[7] LIU Q，MILLE L，VILLALOBOS C，et al. 3D-bioprinted cholangiocarcinoma-on-a-chip model for evaluating drug responses[J]. Bio-Design and Manufacturing，2023，6(4)：373-389.
[8] SUN Xuming，REN Wu，XIE Linyan，et al. Recent advances in 3D bioprinting of tissues and organs for transplantation and drug screening[J]. Virtual and Physical Prototyping，2024，19(1)：e2384662.
[9] HU Yan，ZHU Tong，CUI Haitao，et al. Integrating 3D bioprinting and organoids to better recapitulate the complexity of cellular microenvironments for tissue engineering[J]. Advanced Healthcare Materials，2025，14(3)：2403762.
[10] HUANG M，CHRISTAKOPOULOS F，ROTH J，et al. Organoid bioprinting：From cells to functional tissues[J]. Nature Reviews Bioengineering，2025，3(2)：126-142.
[11] SATO T，VRIES R，SNIPPERT H，et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche[J]. Nature，2009，459(7244)：262-265.
[12] BARKER N，HUCH M，KUJALA P，et al. Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro[J]. Cell Stem Cell，2010，6(1)：25-36.
[13] EIRAKU M，TAKATA N，ISHIBASHI H，et al. Self-organizing optic-cup morphogenesis in three-dimensional culture[J]. Nature，2011，472(7341)：51-56.
[14] LANCASTER M，RENNER M，MARTIN C，et al. Cerebral organoids model human brain development and microcephaly[J]. Nature，2013，501(7467)：373-379.
[15] SHI Min，FU Ping，BONVENTRE J，et al. Directed differentiation of ureteric bud and collecting duct organoids from human pluripotent stem cells[J]. Nature Protocols. 2023，18(8)：2485-2508.
[16] HAN Yuling，DUAN Xiaohua，YANG Liuliu，et al. Identification of SARS-CoV-2 inhibitors using lung and colonic organoids[J]. Nature. 2021，589(7841)：270-275.
[17] MATANO M，DATE S，SHIMOKAWA M，et al. Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids[J]. Nature Medicine. 2015，21(3)：256-262.
[18] YUI S，NAKAMURA T，SATO T，et al. Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell[J]. Nature Medicine，2012，18(4)：618-623.
[19] TAKASATO M，ER P，CHIU H，et al. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis[J]. Nature，2015，526(7574)：564-568.
[20] XIANG Dongxi，HE Aina，ZHOU Rong，et al. Building consensus on the application of organoid-based drug sensitivity testing in cancer precision medicine and drug development[J]. Theranostics，2024，14(8)：3300-3316.
[21] XIE Jundong，YANG Qingyang，ZHANG Yanan，et al. The bioengineering of microspheric skin organoids and their application in drug screening[J]. Advanced Science，2025，12(22)：2416863.
[22] MAROTTA D，IJAZ L，BARBAR L，et al. Effects of microgravity on human iPSC-derived neural organoids on the International Space Station[J]. Stem Cells Translational Medicine，2024，13(12)：1186-1197.
[23] MO Xingwu，ZHANG Yanmei，WANG Zixuan，et al. Satellite-based on-orbit printing of 3D tumor models[J]. Advanced Materials. 2024，36(34)：2309618.
[24] POLAK R，ZHANG E，KUO C. Cancer organoids 2.0：Modelling the complexity of the tumour immune microenvironment[J]. Nature Reviews Cancer，2024，24(8)：523-539.
[25] LI M，IZPISUA B. Organoids — Preclinical models of human disease[J]. New England Journal of Medicine，2019，380(6)：569-579.
[26] AZIZI M S，MOHAVED S，NARAYAN R. Inkjet dispensing technologies：recent advances for novel drug discovery[J]. Expert Opinion on Drug Discovery，2018，14(2)：101-113.
[27] GUPTA D，DERMAN I，XU C，et al. Droplet-based bioprinting[J]. Nature Reviews Methods Primers，2025，5(1)：25.
[28] XU Tao，ZHAO Weixin，ZHU Jianming，et al. Complex heterogeneous tissue constructs containing multiple cell types prepared by inkjet printing technology[J]. Biomaterials，2013，34(1)：130-139.
[29] CHEN Keke，JIANG Erhui，WEI Xiaoyun，et al. The acoustic droplet printing of functional tumor microenvironments[J]. Lab Chip，2021，21(8)：1604-1612.
[30] BHISE N，MANOHARAN V，MASSA S，et al. A liver-on-a-chip platform with bioprinted hepatic spheroids[J]. Biofabrication，2016，8(1)：14101.
[31] CUI Xiaofeng，BOLAND Thomas. Human microvasculature fabrication using thermal inkjet printing technology[J]. Biomaterials，2009，30(31)：6221-6227.
[32] WIJSHOFF H. The dynamics of the piezo inkjet printhead operation[J]. Physics Reports. 2010，491(4-5)：77-177.
[33] UMEZU S，OHMORI H. Characteristics on micro-biofabrication by patterning with electrostatically injected droplet[J]. CIRP Annals，2014，63(1)：221-224.
[34] ONSES M，SUTANTO E，FERREIRA P，et al. Mechanisms，capabilities，and applications of high-resolution electrohydrodynamic jet printing[J]. Small，2015，11(34)：4237-4266.
[35] GU Zeming，FU Jianzhong，LIN Hui，et al. Development of 3D bioprinting：From printing methods to biomedical applications[J]. Asian Journal of Pharmaceutical Sciences，2020，15(5)：529-557.
[36] JEONG Y，YOO J，LEE S，et al. 3D digital light process bioprinting：Cutting-edge platforms for resolution of organ fabrication[J]. Materials Today Bio.，2024，29：101284.
[37] LI Wanlu，WANG Mian，MA Huiling，et al. Stereolithography apparatus and digital light processing-based 3D bioprinting for tissue fabrication[J]. iScience. 2023，26(2)：106039.
[38] WANG Zongjie，JIN Xian，DAI Ru，et al. An ultrafast hydrogel photocrosslinking method for direct laser bioprinting[J]. RSC Advances，2016，6(25)：2114-2199.
[39] GOODARZI H H，DOGAN E，MIRI A，et al. Digital light processing bioprinting advances for microtissue models[J]. ACS Biomaterials Science & Engineering，2022，8(4)：1381-1395.
[40] JING Xian，FU Hongxun，YU Baojun，et al. Two-photon polymerization for 3D biomedical scaffolds：Overview and updates[J]. Frontiers in Bioengineering and Biotechnology，2022，10：994355.
[41] KELLY B，BHATTACHARYA I，HEIDARI H，et al. Volumetric additive manufacturing via tomographic reconstruction[J]. Science，2019，363(6431)：1075-1079.
[42] TOOMBS J，LUITZ M，COOK C，et al. Volumetric additive manufacturing of silica glass with microscale computed axial lithography[J]. Science，2022，376(6590)：308-312.
[43] FAIRBANKS B，SCHWARTZ M，BOWMAN C，et al. Photoinitiated polymerization of PEG-diacrylate with lithium phenyl-2，4，6-trimethylbenzoylphosphinate：Polymerization rate and cytocompatibility[J]. Biomaterials， 2009，30(35)：6702-6707.
[44] MALDA J，VISSER J，MELCHELS F，et al. 25th Anniversary Article：Engineering hydrogels for biofabrication[J]. Advanced Materials，2013，25(36)：5011-5028.
[45] HU Yu，LUO Zhi，BAO Yinyin. Trends in photopolymerization 3D printing for advanced drug delivery applications[J]. Biomacromolecules，2025，26(1)：85-117.
[46] SEXTON Z，RÜTSCHE D，HERRMANN J，et al. Rapid model-guided design of organ-scale synthetic vasculature for biomanufacturing[J]. Science，2025，388(6752)：1198-1204.
[47] DALTON P，WOODFIELD T，MIRONOV V，et al. Advances in hybrid fabrication toward hierarchical tissue constructs[J]. Advanced Science，2020，7(11)：1902953.
[48] MCCORMACK A，HIGHLEY C，LESLIE N，et al. 3D printing in suspension baths：Keeping the promises of bioprinting afloat[J]. Trends in Biotechnology，2020，38(6)：584-593.
[49] LEE A，HUDSON A，SHIWARSKI D，et al. 3D bioprinting of collagen to rebuild components of the human heart[J]. Science (American Association for the Advancement of Science)，2019，365(6452)：482-487.
[50] SKYLAR-SCOTT M，UZEL S，NAM L，et al. Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels[J]. Science advances，2019，5(9)：eaaw2459.
[51] FANG Yongcong，GUO Yihan，WU Bingyan，et al. Expanding embedded 3D bioprinting capability for engineering complex organs with freeform vascular networks[J]. Advanced Materials，2023，35(22)：2205082.
[52] WU Bingyan，ZENG Zhaoxi，FANG Yongcong，et al. Omnidirectional elastic constraint-based embedded 3D printing in non-yield stress fluids for engineering complex tissues[J]. Materials Today，2024，80：74-86.
[53] JENTSCH S，NASEHI R，KUCKELKORN C，et al. Multiscale 3D bioprinting by nozzle-free acoustic droplet ejection[J]. Small Methods，2021，5(6)：2000971.
[54] CAROU-SENRA P，ONG J，CASTRO B，et al. Predicting pharmaceutical inkjet printing outcomes using machine learning[J]. International Journal of Pharmaceutics：X. 2023，5：100181.
[55] HE Chaofan，QIAO Tianhong，WANG Guanghao，et al. High-resolution projection-based 3D bioprinting[J]. Nature Reviews Bioengineering，2025，3(2)：143-158.
[56] TUMBLESTON J，SHIRVANYANTS D，ERMOSHKIN N，et al. Continuous liquid interface production of 3D objects[J]. Science，2015，347(6228)：1349-1352.
[57] VIDLER C，HALWES M，KOLESNIK K，et al. Dynamic interface printing[J]. Nature，2024，634(8036)：1096-1102.
[58] REGEHLY M，GARMSHAUSEN Y，REUTER M，et al. Xolography for linear volumetric 3D printing[J]. Nature，2020，588(7839)：620-624.
[59] RIZZO R，RUETSCHE D，LIU H，et al. Optimized photoclick (bio)resins for fast volumetric bioprinting[J]. Advanced Materials，2021，33(49)：2102900.
[60] GRÖßBACHER G，BARTOLF K M，GERGELY C，et al. Volumetric printing across melt electrowritten scaffolds fabricates multi-material living constructs with tunable architecture and mechanics[J]. Advanced Materials，2023，35(32)：2300756.
[61] RIBEZZI D，GUEYE M，FLORCZAK S，et al. Shaping synthetic multicellular and complex multimaterial tissues via embedded extrusion-volumetric printing of microgels[J]. Advanced Materials，2023，35(36)：2301673.
[62] RIFFE M，DAVIDSON M，SEYMOUR G，et al. Multi-material volumetric additive manufacturing of hydrogels using gelatin as a sacrificial network and 3D suspension bath[J]. Advanced Materials，2024，36(34)：2309026.
[63] LIAN Liming，XIE Maobin，LUO Zeyu，et al. Rapid volumetric bioprinting of decellularized extracellular matrix bioinks[J]. Advanced Materials，2024，36(34)：2304846.
[64] LIU W，ZHANG Y，HEINRICH M，et al. Rapid continuous multimaterial extrusion bioprinting[J]. Advanced Materials，2017，29(3)：1604630.
[65] RAVANBAKHSH H，KARAMZADEH V，BAO G，et al. Emerging technologies in multi-material bioprinting[J]. Advanced Materials，2021，33(49)：2104730.
[66] BROWN N，AMES D，MUELLER J. Multimaterial extrusion 3D printing printheads[J]. Nature Reviews Materials，2025.
[67] WARD K，FAN Z. Mixing in microfluidic devices and enhancement methods[J]. Journal of Micromechanics and Microengineering，2015，25(9)：94001.
[68] IDASZEK J，COSTANTINI M，KARLSEN T，et al. 3D bioprinting of hydrogel constructs with cell and material gradients for the regeneration of full-thickness chondral defect using a microfluidic printing head[J]. Biofabrication，2019，11(4)：44101.
[69] OBER T，FORESTI D，LEWIS J. Active mixing of complex fluids at the microscale[J]. Proceedings of the National Academy of Sciences，2015，112(40)：12293-12298.
[70] BACHMAN H，CHEN C，RUFO J，et al. An acoustofluidic device for efficient mixing over a wide range of flow rates[J]. Lab on a Chip，2020，20(7)：1238-1248.
[71] TAKAGI D，LIN W，MATSUMOTO T，et al. High-precision three-dimensional inkjet technology for live cell bioprinting[J]. International Journal of Bioprinting，2019，5(2)：208.
[72] YAO Kuo，GUO Kai，WANG Heran，et al. Multi-nozzles 3D bioprinting collagen/thermoplastic elasto-mer scaffold with interconnect pores[J]. Micromachines，2025，16(4)：429.
[73] CHENG Ping，WANG Kui，Le DUIGOU Antoine，et al. A novel dual-nozzle 3D printing method for continuous fiber reinforced composite cellular structures[J]. Composites Communications，2023，37：101448.
[74] GAO Ziqi，YIN Jun，LIU Peng，et al. Simultaneous multi-material embedded printing for 3D heterogeneous structures[J]. International Journal of Extreme Manufacturing，2023，5(3)：35001-35498.
[75] ITOH M，NAKAYAMA K，NOGUCHI R，et al. Scaffold-free tubular tissues created by a bio-3D printer undergo remodeling and endothelialization when implanted in rat aortae[J]. PLOS ONE，2015，10(9)：e0136681.
[76] KIM M，SINGH Y，CELIK N，et al. High-throughput bioprinting of spheroids for scalable tissue fabrication[J]. Nature Communications，2024，15(1)：10083.
[77] LI Kai，HUANG Wenhui，GUO Haitao，et al. Advancements in robotic arm-based 3D bioprinting for biomedical applications[J]. Life Med，2023，2(6)：lnad046.
[78] FRITSCHEN A，LINDNER N，SCHOLPP S，et al. High-scale 3D-bioprinting platform for the automated production of vascularized organs-on-a-chip[J]. Advanced Healthcare Materials，2024，13(17)：2304028.
[79] ZHANG Zeyu，WU Chenming，DAI Chengkai，et al. A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication[J]. Bioactive Materials，2022，18：138-150.
[80] JIANG Shengwei，ZHAO Haoran，ZHANG Weijie，et al. An automated organoid platform with inter-organoid homogeneity and inter-patient heterogeneity[J]. Cell Reports Medicine，2020，1(9)：100161.
[81] CAO Yuanxiong，TAN Jiayi，ZHAO Haoran，et al. Bead-jet printing enabled sparse mesenchymal stem cell patterning augments skeletal muscle and hair follicle regeneration[J]. Nature Communications，2022，13(1)：7463.
[82] YANG Haowei，LI Jiawei，ZHENG Yichao，et al. Ultra-small tissue-compatible organoid printer for rapid and controllable modeling of respiratory organoids[J]. Device，2024，2(8)：100420.
[83] SHIN Jaemyung，CHUNG Hyunjae，KUMAR Hitendra，et al. 3D bioprinting of human iPSC-Derived kidney organoids using a low-cost，high-throughput customizable 3D bioprinting system[J]. Bioprinting，2024，38：e00337.
[84] ZHANG Zhenrui，ZHOU Xianhao，FANG Yongcong，et al. AI-driven 3D bioprinting for regenerative medicine：From bench to bedside[J]. Bioactive Materials，2025，45：201-230.
[85] OH D，SHIRZAD M，CHANG K M，et al. Rheology-informed hierarchical machine learning model for the prediction of printing resolution in extrusion-based bioprinting[J]. International Journal of Bioprinting，2023，9(6)：1280.
[86] NADERNEZHAD A，GROLL J. Machine learning reveals a general understanding of printability in formulations based on rheology additives[J]. Advanced Science，2022，9(29)：2202638.
[87] BONATTI A，VOZZI G，CHUA C，et al. A deep learning quality control loop of the extrusion-based bioprinting process[J]. Int J Bioprint，2022，8(4)：620.
[88] SAMPAIO D S C，BOOS J，GOLDOWSKY J，et al. High-throughput platform for label-free sorting of 3D spheroids using deep learning[J]. Frontiers in Bioengineering and Biotechnology，2024，12：1432737.
[89] LAWLOR K，VANSLAMBROUCK J，HIGGINS J，et al. Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation[J]. Nature Materials，2021，20(2)：260-271.
[90] UTAMA R，ATAPATTU L，O'MAHONY A，et al. A 3D bioprinter specifically designed for the high-throughput production of matrix-embedded multicellular spheroids[J]. iScience. 2020，23(10)：101621.
[91] HWANG H，YOU S，MA X，et al. High throughput direct 3D bioprinting in multiwell plates[J]. Biofabrication，2021，13(2).
[92] HE Qiulin，LIAO Youguo，ZHANG Jingwei，et al. “All-in-One” gel system for whole procedure of stem-cell amplification and tissue engineering[J]. Small，2020，16(16)：1906539.
[93] XIE Chang，LIANG Renjie，YE Jinchun，et al. High-efficient engineering of osteo-callus organoids for rapid bone regeneration within one month[J]. Biomaterials，2022，288：121741.
[94] MA Wenping，LU Hongxu，XIAO Yin，et al. Advancing organoid development with 3D bioprinting[J]. Organoid Research，2025，1(1)：25040004.
[95] KIM E，CHOI S，KANG B，et al. Creation of bladder assembloids mimicking tissue regeneration and cancer[J]. Nature，2020，588(7839)：664-669.
[96] WANG Y，GUNASEKARA D，REED M，et al. A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium[J]. Biomaterials，2017，128：44-55.
[97] ZHANG I，CHOI L，FRIEND N，et al. Clickable PEG-norbornene microgels support suspension bioprinting and microvascular assembly[J]. Acta Biomaterialia，2025，201：283-296.
[98] MENG F，MEYER C，JOUNG D，et al. 3D bioprinted in vitro metastatic models via reconstruction of tumor microenvironments[J]. Advanced Materials，2019，31(10)：1806899.
[99] KIM B，CHO W，GAO G，et al. Construction of tissue-level cancer-vascular model with high-precision position control via in situ 3D cell printing[J]. Small Methods，2021，5(7)：2100072.
[100] ANSARI A，BHOWMIK S，ZHANG K，et al. A visible light-responsive hydrogel to study the effect of dynamic tissue stiffness on cellular mechanosensing[J]. Advanced Functional Materials，2025，35(35)：2501585.
[101] NAKAMURA K，Di CAPRIO N，BURDICK J. Engineered shape-morphing transitions in hydrogels through suspension bath printing of temperature- responsive granular hydrogel inks[J]. Advanced Materials，2024，36(47)：2410661.
[102] CHEN Keyu，WANG Jiachen，CAO Jue，et al. Enzyme-responsive microgel with controlled drug release，lubrication and adhesion capability for osteoarthritis attenuation[J]. Acta Biomaterialia，2024，190：191-204.
[103] OUYANG Liliang，ARMSTRONG J，LIN Yiyang，et al. Expanding and optimizing 3D bioprinting capabilities using complementary network bioinks[J]. Science Advances，2020，6(38).
[104] CHANDORKAR Y，BASTARD C，Di RUSSO J，et al. Cells feel the beat – temporal effect of cyclic mechanical actuation on muscle cells[J]. Applied Materials Today，2022，27：101492.
[105] WANG Yongtao，HOU Yan，HAO Tian，et al. Model construction and clinical therapeutic potential of engineered cardiac organoids for cardiovascular diseases[J]. Biomater Transl，2024，5(4)：337-354.
[106] WU Qinghua，XUE Ruikang，ZHAO Yimu，et al. Automated fabrication of a scalable heart-on-a-chip device by 3D printing of thermoplastic elastomer nanocomposite and hot embossing[J]. Bioactive Materials，2024，33：46-60.
[107] LIU Jingjing，LI Jiuzhou，CHEN Yasi，et al. Advances in bone organoids research and future perspectives[J]. Biofabrication，2025，17(4)：40865550.
[108] ZHANG J，GRIESBACH J，GANEYEV M，et al. Long-term mechanical loading is required for the formation of 3D bioprinted functional osteocyte bone organoids[J]. Biofabrication，2022，14(3)：35617929.
[109] FANG Yongcong，JI Mengke，WU Bingyan，et al. Engineering highly vascularized bone tissues by 3D bioprinting of granular prevascularized spheroids[J]. ACS Applied Materials & Interfaces，2023，15(37)：43492-43502.
[110] GEHLEN J，QIU W，SCHÄDLI G，et al. Tomographic volumetric bioprinting of heterocellular bone-like tissues in seconds[J]. Acta Biomaterialia，2023，156：49-60.
[111] LI Wenxiu，ZHOU Zhihang，ZHOU Xiaoyu，et al. 3D biomimetic models to reconstitute tumor microenvironment in vitro：Spheroids，organoids，and tumor-on-a-chip[J]. Advanced Healthcare Materials，2023，12(18)：2202609.
[112] NATH S，DEVI G. Three-dimensional culture systems in cancer research：Focus on tumor spheroid model[J]. Pharmacology & Therapeutics，2016，163：94-108.
[113] JU Mingguang，JIN Zhizhong，YU Xue，et al. Gastric cancer models developed via GelMA 3D bioprinting accurately mimic cancer hallmarks，tumor microenvironment features，and drug responses[J]. Small，2025，21(8)：2409321.
[114] CHOI Y，NA D，YOON G，et al. Prediction of patient drug response via 3D bioprinted gastric cancer model utilized patient-derived tissue laden tissue-specific bioink[J]. Advanced Science，2025，12(10)：2411769.
[115] CHEN Hui，WU Zhuhao，GONG Zhiyi，et al. Acoustic bioprinting of patient-derived organoids for predicting cancer therapy responses[J]. Advanced Healthcare Materials，2022，11(13)：2102784.