Abstract: Stereo light microscope which possesses relatively large depth of field, good performance in real-time and no breaking surface of sample, is used in stereo three-dimensional information measurement. An imaging model for stereo light microscope is developed and its parameter calibration is discussed. Because the number of equation in imaging model is less than the number of parameter, constraint must be considered. A new calibration method combining the symmetry and small difference between light subsystems is developed to resolve stereo light microscope calibration with the approximate conditions. The robustness of parameter estimates, parameter correlations and repeatability are analyzed. At last, a series of calibration standards surfaces are reconstructed in its movement.

Key words: Calibration, Micromanipulation, Reconstruction, Stereo light microscope