Abstract: A noncontact device based on the principle of high potentiometric dispersion technology for cell microencapsulation is designed and manufactured. Sodium alginate is solidified in calcium chloride solution. Integrated microencapsules with smooth surface, regular shape and even size are prepared. Parameters influencing the forming of cell microencapsules like electric field voltage, push speed, electrode distance and syringe needle model are analyzed and optimized. Adipose derived stem cells (ADSCs) microcapsules are prepared via the device. After one-week-long culture, ADSCs demonstrate high viability and significant proliferation.

Key words: Adipose derived stem cells, High potentiometric dispersion, Microencapsulation, Noncontact