Research Progress and Challenges of 3D Printed Conductive Biological Scaffolds

doi:10.3901/JME.2023.23.186

Abstract

Abstract: Tissue engineering provides a new solution for regenerative medicine such as repairing or replacing diseased or damaged tissues. In tissue engineering, biological scaffolds, as one of the key elements (carriers), need to simulate the extracellular matrix environment for cell survival as much as possible. They should not only have biocompatibility, biodegradability and mechanical properties, but also have electrical conductivity to conduct bioelectricity. In addition, 3D printing technology has irreplaceable advantages in the manufacturing of complex bionic structures, and has become one of the most popular technologies in the manufacturing of biological scaffolds. Therefore, 3D printing of conductive biological scaffolds has become one of the hot spots in the research of biological scaffolds. In response to this, it is necessary to sort out and analyze the current contents of 3D printing conductive biological scaffolds. The current research status of conductive biological scaffolds is briefly introduced, including conductive materials, conductive methods, 3D printing methods of conductive biological scaffolds, and their typical applications in nerve cells, bone cells and cardiomyocytes. Finally, the future development direction of conductive biological scaffolds is summarized.

Key words: 3D printing, conductive biological scaffold, conductive materials, cells culture

CLC Number:

TG164

LI Wenhai, ZHANG Guangming, YU Zun, HAN Zhifeng, MA Lingxuan, PENG Zilong, XIAO Miao, XU Lin, XI Yongming, LAN Hongbo. Research Progress and Challenges of 3D Printed Conductive Biological Scaffolds[J]. Journal of Mechanical Engineering, 2023, 59(23): 186-210.

References

[1] BEDIR T，ULAG S，USTUNDAG C B，et al. 3D bioprinting applications in neural tissue engineering for spinal cord injury repair[J]. Materials Science and Engineering:C，2020，110:110741.
[2] MONIA O，MILENA F，ROBERTO D P，et al. Biofabrication and bone tissue regeneration:Cell source，approaches，and challenges[J]. Frontiers in Bioengineering and Biotechnology，2017，5:17.
[3] FUKADA E，YASUDA I. On the piezoelectric effect of bone[J]. Journal of the Physical Society of Japan，1957，12(10):1158-1162.
[4] ALLAHYARI Z，HAGHIGHIPOUR N，MOZTARZADEH F，et al. Optimization of electrical stimulation parameters for MG-63 cell proliferation on chitosan/functionalized multiwalled carbon nanotube films[J]. RSC Advances，2016，6(111):109902.
[5] GHOLIZADEH S，MOZTARZADEH F，HAGHIGHIPOUR N，et al. Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering[J]. International Journal of Biological Macromolecules，2017，97:365-372.
[6] SHAO S ，ZHOU S ，LI L，et al. Osteoblast function on electrically conductive electrospun PLA/MWCNTs nanofibers[J]. Biomaterials，2011，32(11):2821-2833.
[7] RAVICHANDRAN R ，VENUGOPAL J R ，SUNDARRAJAN S，et al. Precipitation of nanohydroxyapatite on PLLA/PBLG/Collagen nanofibrous structures for the differentiation of adipose derived stem cells to osteogenic lineage[J]. Biomaterials，2012，33(3):846-855.
[8] LEVIN M. Large-scale biophysics:ion flows and regeneration[J]. Trends in cell biology，2007，17(6):261-70.
[9] LEVIN M. Bioelectric mechanisms in regeneration:unique aspects and future perspectives[C]//Seminars in Cell & Developmental Biology. Academic Press，2009，20(5):543-556.
[10] LEVIN M. Bioelectromagnetics in morphogenesis[J]. Bioelectromagnetics，2003，24(5):295-315.
[11] PRABHAKARAN P，PALANIYANDI T，KANAGAVALLI B，et al. Prospect and retrospect of 3D bio-printin[J]. Acta Histochemica，2022，124(7):151932.
[12] SUN F，SUN X，WANG H，et al. Application of 3D-printed，PLGA-based scaffolds in bone tissue engineering[J]. International Journal of Molecular Sciences，2022，23(10):5831.
[13] BAIER R V，RAGGIO J I C，GIOVANETTI C M，et al. Shape fidelity，mechanical and biological performance of 3D printed polycaprolactone-bioactive glass composite scaffolds[J]. Biomaterials Advances，2022，134:112540.
[14] GAO C，LU C，JIAN Z，et al. 3D bioprinting for fabricating artificial skin tissue[J]. Colloids and Surfaces B:Biointerfaces，2021，208:112041.
[15] LAIRD N Z，ACRI T M，CHAKKA J L，et al. Applications of nanotechnology in 3D printed tissue engineering scaffolds[J]. European Journal of Pharmaceutics and Biopharmaceutics，2021，161:15-28.
[16] FENG Y，ZHU S，MEI D，et al. Application of 3D printing technology in bone tissue engineering:a review[J]. Current Drug Delivery，2021，18(7):847-861.
[17] SHABBIRAHMED A M，SEKAR R，GOMEZ L A，et al. Recent developments of silk-based scaffolds for tissue engineering and regenerative medicine applications:A special focus on the advancement of 3D printing[J]. Biomimetics，2023，8(1):16.
[18] ZIELIŃSKI P S，GUDETI P K R，RIKMANSPOEL T，et al. 3D printing of bio-instructive materials:Toward directing the cell[J]. Bioactive Materials，2023，19:292-327.
[19] KIRCHMAJER D M，GORKIN III R. An overview of the suitability of hydrogel-forming polymers for extrusion-based 3D-printing[J]. Journal of Materials Chemistry B，2015，3(20):4105-4117.
[20] LIU H，WANG Y，YANG Y，et al. Aligned graphene/silk fibroin conductive fibrous scaffolds for guiding neurite outgrowth in rat spinal cord neurons[J]. Journal of Biomedical Materials Research Part A，2021，109(4):488-499.
[21] SAYYAR S，BJORNINEN M，HAIMI S，et al. UV cross-linkable graphene/poly (trimethylene carbonate) composites for 3D printing of electrically conductive scaffolds[J]. ACS Applied Materials & Interfaces，2016，8(46):31916-31925.
[22] LI J，LIU X，CROOK J M，et al. A 3D printed graphene electrode device for enhanced and scalable stem cell culture，osteoinduction and tissue building[J]. Materials & Design，2021，201:109473.
[23] ESRAFILZADEH D，JALILI R，STEWART E M，et al. High-performance multifunctional graphene-PLGA fibers:Toward biomimetic and conducting 3D scaffolds[J]. Advanced Functional Materials，2016，26(18):3105-3117.
[24] JAKUS A E，SECOR E B，RUTZ A L，et al. Three-dimensional printing of high-content graphene scaffolds for electronic and biomedical applications[J]. ACS Nano，2015，9(4):4636-4648.
[25] WANG J，WANG H，MO X，et al. Reduced graphene oxide-encapsulated microfiber patterns enable controllable formation of neuronal-like networks[J]. Adv. Mater.，2020，32(40):2004555.
[26] LEE S J，ZHU W，NOWICKI M，et al. 3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration[J]. Journal of Neural Engineering，2018，15(1):016018.
[27] KABIRI M，SOLEIMANI M，SHABANI I，et al. Neural differentiation of mouse embryonic stem cells on conductive nanofiber scaffolds[J]. Biotechnology Letters，2012，34(7):1357-65.
[28] HO C M B，MISHRA A，LIN P T P，et al. 3D printed polycaprolactone carbon nanotube composite scaffolds for cardiac tissue engineering[J]. Macromolecular Bioscience，2017，17(4):1600250.
[29] ZHU K，SHIN S R，VAN KEMPEN T，et al. Gold nanocomposite bioink for printing 3D cardiac constructs[J]. Advanced Functional Materials，2017，27(12):1605352.
[30] NAVAEI A，SAINI H，CHRISTENSON W，et al. Gold nanorod-incorporated gelatin-based conductive hydrogels for engineering cardiac tissue constructs[J]. Acta Biomaterialia，2016，41:133-46.
[31] DVIR T，TIMKO B P，BRIGHAM M D，et al. Nanowired three-dimensional cardiac patches[J]. Nature Nanotechnology，2011，6(11):720-5.
[32] RASTIN H，ZHANG B，BI J，et al. 3D printing of cell-laden electroconductive bioinks for tissue engineering applications[J]. Journal of Materials Chemistry B，2020，8(27):5862-5876.
[33] SHU B，SUN X，LIU R，et al. Restoring electrical connection using a conductive biomaterial provides a new therapeutic strategy for rats with spinal cord injury[J]. Neuroscience Letters，2019，692:33-40.
[34] XIE J，MACEWAN M R，WILLERTH S M，et al. Conductive core-sheath nanofibers and their potential application in neural tissue engineering[J]. Advanced Functional Materials，2009，19(14)，2312-2318.
[35] HUANG J，LU L，ZHANG J，et al. Electrical stimulation to conductive scaffold promotes axonal regeneration and remyelination in a rat model of large nerve defect[J]. PLoS One，2012，7(6):e39526.
[36] SHI X，CHANG H，CHEN S，et al. Regulating cellular behavior on few-layer reduced graphene oxide films with well-controlled reduction states[J]. Advanced Functional Materials，2012，22(4):751-759.
[37] LIU Z，ROBINSON J T，SUN X，et al. PEGylated nanographene oxide for delivery of water-insoluble cancer drugs[J]. Journal of the American Chemical Society，2008，130(33):10876-10877.
[38] LA W G，PARK S，YOON H H，et al. Delivery of a therapeutic protein for bone regeneration from a substrate coated with graphene oxide[J]. Small，2013，9(23):4051-4060.
[39] FU C，YANG X，TAN S，et al. Enhancing cell proliferation and osteogenic differentiation of MC3T3-E1 pre-osteoblasts by BMP-2 delivery in graphene oxide-incorporated PLGA/HA biodegradable microcarriers[J]. Scientific Reports，2017，7(1):1-13.
[40] SAYYAR S，OFFICER D L，WALLACE G G. Fabrication of 3D structures from graphene-based biocomposites[J]. Journal of Materials Chemistry B，2017，5(19):3462-3482.
[41] UNAGOLLA J M，JAYASURIYA A C. Enhanced cell functions on graphene oxide incorporated 3D printed polycaprolactone scaffolds[J]. Materials Science and Engineering:C，2019，102:1-11.
[42] FU C，PAN S，MA Y，et al. Effect of electrical stimulation combined with graphene-oxide-based membranes on neural stem cell proliferation and differentiation[J]. Artificial Cells，Nanomedicine，and Biotechnology，2019，47(1):1867-1876.
[43] DEPAN D，GIRASE B，SHAH J，et al. Structure-process-property relationship of the polar graphene oxide-mediated cellular response and stimulated growth of osteoblasts on hybrid chitosan network structure nanocomposite scaffolds[J]. Acta Biomaterialia，2011，7(9):3432-3445.
[44] DUBEY N，BENTINI R，ISLAM I，et al. Graphene:a versatile carbon-based material for bone tissue engineering[J]. Stem Cells International，2015，2015:804213.
[45] CHEN G-Y，PANG D-P，HWANG S-M，et al. A graphene-based platform for induced pluripotent stem cells culture and differentiation[J]. Biomaterials，2012，33(2):418-427.
[46] NALVURAN H，ELçIN A E，ELçIN Y M. Nanofibrous silk fibroin/reduced graphene oxide scaffolds for tissue engineering and cell culture applications[J]. International journal of Biological Macromolecules，2018，114:77-84.
[47] RYU S，KIM B-S. Culture of neural cells and stem cells on graphene[J]. Tissue Engineering and Regenerative Medicine，2013，10(2):39-46.
[48] JASIM D A，LOZANO N，BUSSY C，et al. Graphene-based papers as substrates for cell growth:Characterisation and impact on mammalian cells[J]. FlatChem，2018，12:17-25.
[49] LIAO C，LI Y，TJONG S C. Graphene nanomaterials:Synthesis，biocompatibility，and cytotoxicity[J]. International Journal of Molecular Sciences，2018，19(11):3564.
[50] LEE W C，LIM C H，SU C，et al. Cell-assembled graphene biocomposite for enhanced chondrogenic differentiation[J]. Small，2015，11(8):963-9.
[51] KIM J，CHOI K S，KIM Y，et al. Bioactive effects of graphene oxide cell culture substratum on structure and function of human adipose-derived stem cells[J]. J. Biomed. Mater. Res. A，2013，101(12):3520-30.
[52] FABBRO A，SUCAPANE A，TOMA F M，et al. Adhesion to carbon nanotube conductive scaffolds forces action-potential appearance in immature rat spinal neurons[J]. PloS one，2013，8(8):e73621.
[53] KABIRI M，ORAEE-YAZDANI S，DODEL M，et al. Cytocompatibility of a conductive nanofibrous carbon nanotube/poly (L-Lactic acid) composite scaffold intended for nerve tissue engineering[J]. EXCLI Journal，2015，14:851.
[54] SUBRAMANI K，PANDRUVADA S，PULEO D，et al. In vitro evaluation of osteoblast responses to carbon nanotube-coated titanium surfaces[J]. Progress in Orthodontics，2016，17(1):1-9.
[55] URSU E-L，GAVRIL G，MORARIU S，et al. Single-walled carbon nanotubes-G-quadruplex hydrogel nanocomposite matrixes for cell support applications[J]. Materials Science and Engineering:C，2020，111:110800.
[56] LEE J-R，RYU S，KIM S，et al. Behaviors of stem cells on carbon nanotube[J]. Biomaterials Research，2015，19(1):1-6.
[57] HIRATA E，UO M，TAKITA H，et al. Multiwalled carbon nanotube-coating of 3D collagen scaffolds for bone tissue engineering[J]. Carbon，2011，49(10):3284-91.
[58] ALI-BOUCETTA H，AL-JAMAL K T，KOSTARELOS K. Cytotoxic assessment of carbon nanotube interaction with cell cultures[M]. Biomedical Nanotechnology，2011，726:299-312.
[59] AOKI N，AKASAKA T，WATARI F，et al. Carbon nanotubes as scaffolds for cell culture and effect on cellular functions[J]. Dental Materials Journal，2007，26(2):178-185.
[60] ZHAO G，ZHANG X，LI B，et al. Solvent-free fabrication of carbon nanotube/silk fibroin electrospun matrices for enhancing cardiomyocyte functionalities[J]. ACS Biomaterials Science & Engineering，2020，6(3):1630-1640.
[61] ZHOU J，VIJAYAVENKATARAMAN S. 3D-printable conductive materials for tissue engineering and biomedical applications[J]. Bioprinting，2021，24:e00166.
[62] MIHIC A，CUI Z，WU J，et al. A conductive polymer hydrogel supports cell electrical signaling and improves cardiac function after implantation into myocardial infarct[J]. Circulation，2015，132(8):772-84.
[63] ATEH D D，VADGAMA P，NAVSARIA H A. Culture of human keratinocytes on polypyrrole-based conducting polymers[J]. Tissue Engineering，2006，12(4):645-55.
[64] YANG S，JANG L，KIM S，et al. Polypyrrole/alginate hybrid hydrogels:electrically conductive and soft biomaterials for human mesenchymal stem cell culture and potential neural tissue engineering applications[J]. Macromolecular Bioscience，2016，16(11):1653-61.
[65] ZAREI M，SAMIMI A，KHORRAM M，et al. Fabrication and characterization of conductive polypyrrole/chitosan/collagen electrospun nanofiber scaffold for tissue engineering application[J]. International Journal of Biological Macromolecules，2021，168:175-86.
[66] HU W-W，HSU Y-T，CHENG Y-C，et al. Electrical stimulation to promote osteogenesis using conductive polypyrrole films[J]. Materials Science and Engineering:C，2014，37:28-36.
[67] WENG B，LIU X，SHEPHERD R，et al. Inkjet printed polypyrrole/collagen scaffold:A combination of spatial control and electrical stimulation of PC12 cells[J]. Synthetic Metals，2012，162(15-16):1375-80.
[68] QI F，WANG Y，MA T，et al. Electrical regulation of olfactory ensheathing cells using conductive polypyrrole/chitosan polymers[J]. Biomaterials，2013，34(7)，1799-809.
[69] JOUNG D ，TRUONG V ，NEITZKE C C ，et al. Spinal cord scaffolds:3D printed stem-cell derived neural progenitors generate spinal cord scaffolds[J]. Advanced Functional Materials，2018，28(39):1801850.
[70] QIAN Y，ZHAO X，HAN Q，et al. An integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration[J]. Nature Communications，2018，9(1):1-16.
[71] KOFFLER J，ZHU W，QU X，et al. Biomimetic 3D-printed scaffolds for spinal cord injury repair [J]. Nature Medicine，2019，25(2):263-269.
[72] LIU X，HAO M，CHEN Z，et al. 3D bioprinted neural tissue constructs for spinal cord injury repair[J]. Biomaterials，2021，272:120771.
[73] 赵佳伟，兰红波，杨昆，等. 电场驱动熔融喷射沉积高分辨率3D打印[J]. 工程科学学报，2019，41(5):652-661. ZHAO Jiawei，LAN Hongbo，YANG Kun，et al Electric field driven melt spray deposition high resolution 3D printing[J] Journal of Engineering Science，2019，41(5):652-661.
[74] 杨昆，杨建军，赵佳伟，等. 基于电场驱动熔融喷射3D打印大面积聚甲基丙烯酸甲酯微结构制造方法[J]. 高分子材料科学与工程，2019，35(10):115-123. YANG Kun，YANG Jianjun，ZHAO Jiawei，et al. Fabrication of large-area poly (methyl methacrylate) microstructures based on Electric Field Driven Melt Jet 3D Printing[J]. Polymer Materials Science and Engineering，2019，35(10):115-123.
[75] 杨昆，张广明，李晓强，等. 基于电场驱动熔融喷射聚合物基复合材料高分辨率3D打印[J]. 机械工程学报，2021，56(23):193-202. YANG Kun，ZHANG Guangming，LI Xiaoqiang，et al. High resolution 3D printing of polymer matrix composites driven by electric field[J]. Journal of Mechanical Engineering，2020，56(23):193-202.
[76] LI K，WANG D，ZHAO K，et al. Electrohydrodynamic jet 3D printing of PCL/PVP composite scaffold for cell culture[J]. Talanta，2020，211:120750.
[77] HE J，XIA P，LI D. Development of melt electrohydrodynamic 3D printing for complex microscale poly (ε-caprolactone) scaffolds[J]. Biofabrication，2016，8(3):035008.
[78] HE J，ZHANG B，LI Z，et al. High-resolution electrohydrodynamic bioprinting:a new biofabrication strategy for biomimetic micro/nanoscale architectures and living tissue constructs[J]. Biofabrication，2020，12(4):042002.
[79] VIJAYAVENKATARAMAN S，THAHARAH S，ZHANG S，et al. 3D-printed PCL/rGO conductive scaffolds for peripheral nerve injury repair[J]. Artificial Organs，2019，43(5):515-523.
[80] VIJAYAVENKATARAMAN S，THAHARAH S，ZHANG S，et al. Electrohydrodynamic jet 3D-printed PCL/PAA conductive scaffolds with tunable biodegradability as nerve guide conduits (NGCs) for peripheral nerve injury repair[J]. Materials & Design，2019，162:171-184.
[81] VIJAYAVENKATARAMAN S，KANNAN S，CAO T，et al. 3D-printed PCL/PPy conductive scaffolds as three-dimensional porous nerve guide conduits (NGCs) for peripheral nerve injury repair[J]. Frontiers in Bioengineering and Biotechnology，2019，7:266.
[82] PIIRONEN K，HAAPALA M，TALMAN V，et al. Cell adhesion and proliferation on common 3D printing materials used in stereolithography of microfluidic devices[J]. Lab on a Chip，2020，20(13):2372-2382.
[83] AGGAS J R，ABASI S，PHIPPS J F，et al. Microfabricated and 3D printed electroconductive hydrogels of PEDOT:PSS and their application in bioelectronics[J]. Biosensors and Bioelectronics，2020，168:112568.
[84] WU Y，CHEN Y X，YAN J，et al. Fabrication of conductive gelatin methacrylate-polyaniline hydrogels[J]. Acta Biomaterialia，2016，33:122-130.
[85] HEO D N，LEE S-J，TIMSINA R，et al. Development of 3D printable conductive hydrogel with crystallized PEDOT:PSS for neural tissue engineering[J]. Materials Science and Engineering:C，2019，99:582-590.
[86] ZEIN I，HUTMACHER D W，TAN K C，et al. Fused deposition modeling of novel scaffold architectures for tissue engineering applications[J]. Biomaterials，2002，23(4):1169-1185.
[87] HOUSHYAR S，PILLAI M M，SAHA T，et al. Three-dimensional directional nerve guide conduits fabricated by dopamine-functionalized conductive carbon nanofibre-based nanocomposite ink printing[J]. RSC Advances，2020，10(66):40351-40364.
[88] HAN X，YANG D，YANG C，et al. Carbon fiber reinforced PEEK composites based on 3D-printing technology for orthopedic and dental applications[J]. Journal of Clinical Medicine，2019，8(2):240.
[89] DIAS D，VALE A C，CUNHA E P，et al. 3D-printed cryomilled poly (ε-caprolactone)/graphene composite scaffolds for bone tissue regeneration[J]. Journal of Biomedical Materials Research Part B:Applied Biomaterials，2021，109(7):961-972.
[90] GAO Q，NIU X，SHAO L，et al. 3D printing of complex GelMA-based scaffolds with nanoclay[J]. Biofabrication，2019，11(3):035006.
[91] CHOI W J，HWANG K S，KWON H J，et al. Rapid development of dual porous poly (lactic acid) foam using fused deposition modeling (FDM) 3D printing for medical scaffold application[J]. Materials Science and Engineering:C，2020，110:110693.
[92] CERETTI E，GINESTRA P，NETO P，et al. Multi-layered scaffolds production via fused deposition modeling (FDM) using an open source 3D printer:Process parameters optimization for dimensional accuracy and design reproducibility[J]. Procedia CIRP，2017，65:13-8.
[93] YUK H，LU B，LIN S，et al. 3D printing of conducting polymers[J]. Nature Communications，2020，11(1):1-8.
[94] SAYYAR S，GAMBHIR S，CHUNG J，et al. 3D printable conducting hydrogels containing chemically converted graphene[J]. Nanoscale，2017，9(5):2038-2050.
[95] ZHANG G，LAN H，QIAN L，et al. A microscale 3D printing based on the electric-field-driven jet[J]. 3D Printing and Additive Manufacturing，2020，7(1):37-44.
[96] HUANG H，ZHANG G，LI W，et al. The theoretical model and verification of electric-field-driven jet 3D printing for large-height and conformal micro/nano-scale parts[J]. Virtual and Physical Prototyping，2023，18(1):e2140440.
[97] VIJAYAVENKATARAMAN S，ZHANG S，LU W F，et al. Electrohydrodynamic-jetting (EHD-jet) 3D-printed functionally graded scaffolds for tissue engineering applications[J]. Journal of Materials Research，2018，33(14):1999-2011.
[98] LIU H，VIJAYAVENKATARAMAN S，WANG D，et al. Influence of electrohydrodynamic jetting parameters on the morphology of PCL scaffolds[J]. International Journal of Bioprinting，2017，3(1):009.
[99] SUN J，VIJAYAVENKATARAMAN S，LIU H. An overview of scaffold design and fabrication technology for engineered knee meniscus[J]. Materials，2017，10(1):29.
[100] WANG H，VIJAYAVENKATARAMAN S，WU Y，et al. Investigation of process parameters of electrohydro- dynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries[J]. International Journal of Bioprinting，2016，2(1):9.
[101] GAO D，ZHOU J G. Designs and applications of electrohydrodynamic 3D printing[J]. International Journal of Bioprinting，2019，5(1):172.
[102] POELLMANN M J，JOHNSON A J W. Multimaterial polyacrylamide:fabrication with electrohydrodynamic jet printing，applications，and modeling[J]. Biofabrication，2014，6(3):035018.
[103] MIN S-Y，KIM T-S，KIM B J，et al. Large-scale organic nanowire lithography and electronics[J]. Nature Communications，2013，4(1):1-9.
[104] MELCHELS F P，FEIJEN J，GRIJPMA D W. A review on stereolithography and its applications in biomedical engineering[J]. Biomaterials，2010，31(24):6121-6130.
[105] 王晓晶，涂龙，罗晓亮，等. 聚合物基材料4D打印研究进展[J]. 材料导报，2022，36(14):20100265-15. WANG Xiaojing，TU Long，LUO Xiaoliang，et al Research progress of 4D printing of polymer based materials[J]. Material Guide，2022，36(14):20100265-15.
[106] ZHANG G，SONG D，JIANG J，et al. Electrically assisted continuous vat photopolymerization 3D printing for fabricating high-performance ordered graphene/polymer composites[J]. Composites Part B:Engineering，2023，250:110449.
[107] SKOOG S A，GOERING P L，NARAYAN R J. Stereolithography in tissue engineering[J]. Journal of Materials Science:Materials in Medicine，2014，25(3):845-856.
[108] MUERZA-CASCANTE M L，SHOKOOHMAND A，KHOSROTEHRANI K，et al. Endosteal-like extracellular matrix expression on melt electrospun written scaffolds[J]. Acta Biomaterialia，2017，52:145-158.
[109] HUANG J，CHEN Q，JIANG H，et al. A survey of design methods for material extrusion polymer 3D printing[J]. Virtual and Physical Prototyping，2020，15(2):148-162.
[110] AZAD M A，OLAWUNI D，KIMBELL G，et al. Polymers for extrusion-based 3D printing of pharmaceuticals:A holistic materials–process perspective[J]. Pharmaceutics，2020，12(2):124.
[111] LIU Z，WANG Y，WU B，et al. A critical review of fused deposition modeling 3D printing technology in manufacturing polylactic acid parts[J]. The International Journal of Advanced Manufacturing Technology，2019，102(9):2877-2889.
[112] ZAMANI F，AMANI-TEHRAN M，ZAMINY A，et al. Conductive 3D structure nanofibrous scaffolds for spinal cord regeneration[J]. Fibers and Polymers，2017，18(10):1874-1881.
[113] KIYOTAKE E A，MARTIN M D，DETAMORE M S. Regenerative rehabilitation with conductive biomaterials for spinal cord injury[J]. Acta Biomaterialia，2022，139:43-64.
[114] GHASEMI-MOBARAKEH L，PRABHAKARAN M P，MORSHED M，et al. Electrical stimulation of nerve cells using conductive nanofibrous scaffolds for nerve tissue engineering[J]. Tissue Engineering Part A，2009，15(11):3605-3619.
[115] SUDWILAI T，NG J J，BOONKRAI C，et al. Polypyrrole-coated electrospun poly (lactic acid) fibrous scaffold:effects of coating on electrical conductivity and neural cell growth[J]. Journal of Biomaterials Science，Polymer Edition，2014，25(12):1240-1252.
[116] LEE J Y，BASHUR C A，GOLDSTEIN A S，et al. Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications[J]. Biomaterials，2009，30(26):4325-4335.
[117] FARKHONDEHNIA H，AMANI TEHRAN M，ZAMANI F. Fabrication of biocompatible PLGA/PCL/PANI nanofibrous scaffolds with electrical excitability[J]. Fibers and Polymers，2018，19(9):1813-1819.
[118] GOMEZ N，SCHMIDT C E. Nerve growth factor-immobilized polypyrrole:Bioactive electrically conducting polymer for enhanced neurite extension[J]. Journal of Biomedical Materials Research Part A，2007，81(1):135-149.
[119] KHORSHIDI S，KARKHANEH A. Hydrogel/fiber conductive scaffold for bone tissue engineering[J]. Journal of Biomedical Materials Research Part A，2018，106(3):718-724.
[120] WIBOWO A，VYAS C，COOPER G，et al. 3D printing of polycaprolactone-polyaniline electroactive scaffolds for bone tissue engineering[J]. Materials，2020，13(3):512.
[121] GONCALVES E M，OLIVEIRA F J，SILVA R F，et al. Three-dimensional printed PCL-hydroxyapatite scaffolds filled with CNT s for bone cell growth stimulation[J]. Journal of Biomedical Materials Research Part B:Applied Biomaterials，2016，104(6):1210-1219.
[122] CHEN M-C，SUN Y-C，CHEN Y-H. Electrically conductive nanofibers with highly oriented structures and their potential application in skeletal muscle tissue engineering[J]. Acta Biomaterialia，2013，9(3):5562-72.
[123] RAJZER I，ROM M，MENASZEK E，et al. Conductive PANI patterns on electrospun PCL/gelatin scaffolds modified with bioactive particles for bone tissue engineering[J]. Materials Letters，2015，138:60-63.
[124] HARDY J G，VILLANCIO-WOLTER M K，SUKHAVASI R C，et al. Electrical stimulation of human mesenchymal stem cells on conductive nanofibers enhances their differentiation toward osteogenic outcomes[J]. Macromolecular Rapid Communications，2015，36(21):1884-1890.
[125] DE CASTRO J G，RODRIGUES B V，RICCI R，et al. Designing a novel nanocomposite for bone tissue engineering using electrospun conductive PBAT/polypyrrole as a scaffold to direct nanohydroxyapatite electrodeposition[J]. RSC Advances，2016，6(39):32615-32623.
[126] BAI R G，NINAN N，MUTHOOSAMY K，et al. Graphene:A versatile platform for nanotheranostics and tissue engineering[J]. Progress in Materials Science，2018，91:24-69.
[127] SHEVACH M，MAOZ B M，FEINER R，et al. Nanoengineering gold particle composite fibers for cardiac tissue engineering[J]. Journal of Materials Chemistry B，2013，1(39):5210-5217.
[128] FLEISCHER S，SHEVACH M，FEINER R，et al. Coiled fiber scaffolds embedded with gold nanoparticles improve the performance of engineered cardiac tissues[J]. Nanoscale，2014，6(16):9410-9414.
[129] RAVICHANDRAN R，SRIDHAR R，VENUGOPAL J R，et al. Gold nanoparticle loaded hybrid nanofibers for cardiogenic differentiation of stem cells for infarcted myocardium regeneration[J]. Macromolecular Bioscience，2014，14(4):515-525.
[130] LI M ，GUO Y ，WEI Y ， et al. Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications[J]. Biomaterials，2006，27(13):2705-2715.
[131] KAI D，PRABHAKARAN M P，JIN G，et al. Polypyrrole-contained electrospun conductive nanofibrous membranes for cardiac tissue engineering[J]. Journal of Biomedical Materials Research Part A，2011，99(3):376-385.
[132] GELMI A，ZHANG J，CIESLAR-POBUDA A，et al. Electroactive 3D materials for cardiac tissue engineering[J]. Proceedings of SPIE-The International Society for Optical Engineering，2015，9430:445-451.
[133] GELMI A，CIESLAR-POBUDA A，DE MUINCK E，et al. Direct mechanical stimulation of stem cells:A beating electromechanically active scaffold for cardiac tissue engineering[J]. Advanced Healthcare Materials，2016，5(12):1471-1480.
[134] BORRIELLO A，GUARINO V，SCHIAVO L，et al. Optimizing PANi doped electroactive substrates as patches for the regeneration of cardiac muscle[J]. Journal of Materials Science:Materials in Medicine，2011，22(4):1053-1062.